Date of Completion

Spring 5-1-2023

Thesis Advisor(s)

Kazunori Hoshino

Honors Major

Biomedical Engineering

Disciplines

Bioimaging and Biomedical Optics

Abstract

The focus of the following paper is the development of a light sheet or selective plane illumination microscopy (LSM / SPIM) platform to support long duration three dimensional imaging of various cells and tissues. This paper describes the complete design and fabrication process behind the prototype, including a definition of light sheet microscopy and its differences from confocal microscopy, as well as existing open source selective plane microscopy literature and designs. The complete engineering design process as applied to the microscope design is described in detail. Specifically, this paper will focus on the development of an incubation chamber for light sheet imaging. We propose two iterations of an incubation design, and critically evaluate each. We also propose future improvements in controls, namely via the implementation of fuzzy logic control. As the project is not yet complete, further steps and future goals are also described in detail, namely highlighting the integration of a similar incubation chamber in a light sheet microscopy based, dynamic mechanical analysis instrument.

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