Authors

Mita KaleFollow

Date of Completion

Spring 5-31-2021

Thesis Advisor(s)

Daniel Bolnick

Honors Major

Ecology and Evolutionary Biology

Disciplines

Other Ecology and Evolutionary Biology | Parasitology

Abstract

This study was conducted to determine if the monoclonal antibody drug infliximab could effectively suppress the fibrosis immune response in three-spine stickleback fish. Successful suppression of this response could allow for further study of cestode infection and growth without the presence of fibrosis. Infliximab’s efficacy was investigated through conducting two intraperitoneal injection experiments and examinations of the fibrosis in the body cavity of the euthanized stickleback. We used immune adjuvant alum to induce a fibrosis response without the presence of the S. solidus tapeworm parasite. Ultimately, the differences in fibrosis levels between the treatment groups that received alum and the groups that received alum+infliximab were not statistically significant. These findings suggest that infliximab is not a good candidate for attenuating the fibrosis response in three-spine stickleback and other methods of suppressing fibrosis to examine cestode growth should be pursued.

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