Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Flow cytometry analysis of propidium iodide (PI) stained nuclei isolated from leaf tissues was used to estimate the genome size of American ginseng (Panax quinquefolius L.) and evaluate inhibitor y effects of its secondary compounds on PI intercalation. American ginseng nuclear DNA content was estimated to be 10.05 ± 0.04 pg/2C, and therefore its haploid (1C) genome size is 4914 Mbp. There was no significant inhibition of PI fluorescence of reference standard nuclei co-processed with American ginseng. This indicates that secondary compounds of American ginseng do not interfere with PI intercalation. By comparison, the genome size of American ginseng is 31.30 and 10.03 times bigger than that of Arabidopsis thaliana and Oryza sativa , respectively, and is 1.58 times bigger than that of its sister species Panax ginseng C.A. Meyer (Asian ginseng). The large genome size of American ginseng will have valuable implications on its sequencing, structural and functional genomics research

Comments

Originally published in :

Journal of Medicinal Plants Research Vol.6(32) , pp. 4719-4723 , August 2012 DOI: 10.5897/JMPR11.221 ISSN 1996-0875

Copyright © 2012 Author(s) retain the copyright of this article Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License

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