Date of Completion
5-8-2014
Embargo Period
5-8-2014
Keywords
Stress, Exercise, Heat, Dehydration, Immune, cytokine, HSP70, NFAT5, HSF1, eHSP70
Major Advisor
Carl M. Maresh, PhD
Associate Advisor
Elaine C. Lee, PhD
Associate Advisor
Jeff S. Volek, PhD
Associate Advisor
William J. Kraemer, PhD
Associate Advisor
Lawrence E. Armstrong, PhD
Field of Study
Kinesiology
Degree
Doctor of Philosophy
Open Access
Open Access
Abstract
Background: Global changes in immune cells and circulating molecules might indicate whole body stress magnitude. Heat shock protein 70kD (HSP70) and plasma HSP70 might protect through different mechanisms. HSP70 transcription factors, Heat Shock Factor 1 (HSF1) and Nuclear Factor of Activated T-cells 5 (NFAT5) respond to heat and dehydration stress, respectively, and might quantify specific stress exposures. Purpose: Characterize circulating and cellular markers indicating cytoprotective responses following acute stress and recovery in humans. Methods: Laboratory: 36 healthy males completed: 1) "Baseline" followed by 16h fluid deprivation, 2) "Pre" and "Post" 2h cycling (~38°C, ~40%RH), 3) partial rehydration measured "Post1h", 4) and complete recovery ("Recov") upon 24h. Peripheral Blood Mononuclear Cells (PBMCs) were measured for HSP70, HSF1, and NFAT5, and plasma HSP70. Field: 22 healthy males at the 2012 Ironman World Championship. Analyses included Complete Blood Count (CBC) with differential, plasma cytokines, and plasma HSP70 at "Base", "Post", "Post1d", and "Post2d". Athletes who lost least (n=8; Euhy) and most (n=8; Dehy) body mass during the race examined dehydration influences in all variables. Results: Laboratory: PBMCs expressed greater HSP70 at all time points above Base, and at Recov above Pre (pPost2d; p=0.002). More neutrophils (L; p=0.018; %; p=0.030) and nearly less lymphocytes (%; p=0.063) were observed in Dehy than Euhy. Greater IL-10, IL-6 and IL-8 existed at Post than all time points (pConclusion: Whole body stress stimulated an acute inflammatory state in PBMCs and circulating markers. Cellular versus plasma HSP70 exhibited distinct responses, and NFAT5 might indicate whole body dehydration. Understanding cellular mediators might permit greater stress expsure resistance.
Recommended Citation
Munoz, Colleen, "Whole Body Stress and Immune Cell Responses to Exercise, Heat and Dehydration in the 2012 Ironman World Championship and Controlled Laboratory Study" (2014). Doctoral Dissertations. 398.
https://digitalcommons.lib.uconn.edu/dissertations/398