Date of Completion
1-8-2019
Embargo Period
1-16-2020
Keywords
chromatin interaction, super-resolution imaging, ChIA-PET, breast cancer, metastasis, enhancer
Major Advisor
Yijun Ruan
Associate Advisor
Blanka Rogina
Associate Advisor
Derya Unutmaz
Associate Advisor
James Li
Associate Advisor
Jeff Chuang
Field of Study
Biomedical Science
Degree
Doctor of Philosophy
Open Access
Open Access
Abstract
Higher-order chromatin organization in cell nucleus is mysterious. Microscopy and high-throughput sequencing technologies have been applied to reveal the connections between genome structure and gene transcription, which is the main topic of my thesis. Higher-order chromatin organization differs across cell types and species, giving an insight into disease genesis and tumorigenesis. Despite an enormous progress has been made recently in epigenomic study, lots of things remain unknown.
My dissertation reviews the current understanding of epigenomics, characterizes chromatin interaction in mammalian cells using different technologies, investigates chromatin reorganization in breast cancer cells, analyzes integrated genomic and epigenomic data in breast cancer metastasis and discusses results and future directions.
Recommended Citation
Zhu, Jufen, "Characterization of Chromatin Interaction in Mammalian Cells" (2019). Doctoral Dissertations. 2053.
https://digitalcommons.lib.uconn.edu/dissertations/2053