Date of Completion

Spring 5-1-2015

Thesis Advisor(s)

Guillermo Risatti

Honors Major



The purpose of this research is to develop a method for assessing in vivo protein-protein interactions in cultured cells that are permissive to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection. The Non Structural Protein 3 (NSp3) expressed by PRRSV was used as a prey to capture interacting cellular proteins in protein cell lysates using a Pull-Down assay. Interacting proteins were resolved by SDS-PAGE, extracted and identified by means of mass spectrometry. Different alternatives have been tested to create the optimal conditions for performing the technique that will allow further confirmation of virus-host cells protein-protein interaction in PRRSV permissive cells.