Date of Completion
Spring 5-1-2024
Thesis Advisor(s)
Guillermo Risatti
Honors Major
Pathobiology
Disciplines
Animal Diseases | Bacteria | Bacterial Infections and Mycoses | Bacteriology | Bioinformatics | Investigative Techniques | Other Public Health | Pathogenic Microbiology
Abstract
Bacteria in the Pasteurellaceae family are known to cause disease in both human and animal species. Like all species of bacteria, the pathogens in the Pasteurellaceae family evolve and acquire antimicrobial-resistant traits. Antimicrobial resistance of bacteria is a significant public health concern, as treatment of resistant pathogens becomes more difficult, if not impossible, with current medical capabilities. In this project, phenotypic and genotypic analyses were performed on Pasteurellaceae family isolates sourced from the Connecticut Veterinary Medical Diagnostic Laboratory. Phenotypic antibiotic sensitivity patterns of each isolate were assessed against a panel of antibiotics through a Kirby-Bauer disk diffusion test. Disk diffusion tests indicated some antimicrobial resistance to penicillin and tetracycline along with susceptibility to amphenicols and cephalosporins. DNA extraction and sequencing of isolates identified genes known to produce resistance to aminoglycosides, sulfonamides, and tetracycline.
Recommended Citation
Barron, Jillian, "Analysis of Antibiotic Sensitivity Patterns in Pasteurellaceae Family Isolates: A Microbial and Molecular Investigation" (2024). Honors Scholar Theses. 990.
https://digitalcommons.lib.uconn.edu/srhonors_theses/990