Date of Completion
Spring 5-1-2024
Thesis Advisor(s)
Nicholas Leadbeater, James Rusling
Honors Major
Chemistry
Disciplines
Analytical, Diagnostic and Therapeutic Techniques and Equipment | Diagnosis | Inorganic Chemistry
Abstract
This thesis outlines the research conducted over the past two years on the production and application of microfluidic devices (MFDs) in electrochemiluminescent-based bioanalytical assays. The work is categorized into two main projects: designing and manufacturing MFDs and developing electrochemiluminescent (ECL) assays for detecting Alzheimer's disease (AD) associated microRNAs (miRNAs) using CRISPR technology. The process of learning to design MFDs involved acquiring proficiency in computer-aided design (CAD) software, stereolithography (SLA) 3D printing, and iterative design techniques. The development of the ECL-based assay for AD miRNA was a multidisciplinary endeavor, combining elements of inorganic and biological chemistry. Although the research on the development of this assay is still ongoing, this thesis documents the construction, functionalization, and testing processes in detail. It explores the effectiveness of the assay and discusses the optimizations and modifications implemented based on the data collected during testing.
Recommended Citation
Clement, Oscar, "3D-Printed Microfluidic Devices for Electrochemiluminescence Detection of miRNA" (2024). Honors Scholar Theses. 1007.
https://digitalcommons.lib.uconn.edu/srhonors_theses/1007