Date of Completion


Embargo Period



Dr. Paulo Verardi and Dr. Guillermo Risatti

Field of Study



Master of Science

Open Access

Open Access


The host protein cyclophilin A (CypA) has been shown to be necessary for viral replication of several viruses, including human immunodeficiency virus and hepatitis C virus. Previous research done in the Geary laboratory, in collaboration with Pacific Northwest National Laboratory, reported that cyclophilin A remains highly associated with vaccinia virus virions even after successive rounds of purification. The goal of this research is to further investigate how cyclophilin A affects vaccinia virus replication in vitro using primary tissues harvested from CypA (-/-) knockout mice to determine the difference in viral growth kinetics. Results showed different viral growth kinetics in different primary tissues. These results indicate that vaccinia virus is capable of replicating in CypA negative cells, but that there may be tissue specific differences that could play a role in in vivo pathogenesis.

Major Advisor

Dr. Steven Geary